High molecular weight hyaluronic acid vectorised with clay provides long-term hydration and reduces skin brightness.

BACKGROUND: Hyaluronic acid (HA) is a widely used active cosmetic ingredient. Its multiple skin care benefits are modulated by its molecular weight. Low molecular weight (LMW) HA can penetrate the skin, but high molecular weight (HMW) HA remains at the surface. Here, we assessed how vectorization of HMW HA with bentonite clay-achieved with an innovative technology-enhances its cosmetic and hydrating properties. MATERIALS AND METHODS: The two HA forms were applied to skin explants; their penetration and smoothing effects were monitored by Raman spectroscopy and scanning electron microscopy. The two forms were biochemically characterised by chromatography, enzyme sensitivity assays, and analysis of Zeta potential. Cosmetics benefits such as, the smoothing effect of vectorised-HA was assessed in ex vivo experiments on skin explants. A placebo-controlled clinical study was finally conducted applying treatments for 28 days to analyse the final benefits in crow's feet area. RESULTS: Raman spectroscopy analysis revealed native HMW HA to accumulate at the surface of skin explants, whereas vectorised HMW HA was detected in deeper skin layers. This innovative vectorisation process changed the zeta potential of vectorised HMW HA, being then more anionic and negative without impacting the biochemical structure of native HA. In terms of cosmetic benefits, following application of vectorised HMW HA ex vivo, the skin's surface was visibly smoother. This smoothing was clinically confirmed, with a significant reduction in fine lines. CONCLUSION: The development of innovative process vectorising HMW HA allowed HMW HA penetration in the skin. This enhanced penetration extends the clinical benefits of this iconic cosmetic ingredient.

[Determination of 24 elements migration in Yixing clay pottery by inductively coupled plasma mass spectrometry].

OBJECTIVE: To establish an analytical method for determining the migration of 24 elements in Yixing clay pottery in 4% acetic acid simulated solution by inductively coupled plasma mass spectrometry. METHODS: Four types of Yixing clay pottery, including Yixing clay teapot, Yixing clay kettle, Yixing clay pot, and Yixing clay electric stew pot, were immersed in 4% acetic acid as a food simulant for testing. The migration amount of 24 elements in the migration solution was determined using inductively coupled plasma mass spectrometry. RESULTS: Lithium, magnesium, aluminum, iron, and barium elements with a mass concentration of 1000 µg/L; Lead, cadmium, total arsenic, chromium, nickel, copper, vanadium, manganese, antimony, tin, zinc, cobalt, molybdenum, silver, beryllium, thallium, titanium, and strontium elements within 100 µg/L there was a linear relationship within, the r value was between 0.998 739 and 0.999 989. Total mercury at 5.0 µg/L, there was a linear relationship within, the r value of 0.995 056. The detection limit of the elements measured by this method was between 0.5 and 45.0 µg/L, the recovery rate was 80.6%-108.9%, and the relative standard deviation was 1.0%-4.8%(n=6). A total of 32 samples of four types of Yixing clay pottery sold on the market, including teapots, boiling kettles, casseroles, and electric stewing pots, were tested. It was found that the migration of 16 elements, including beryllium, titanium, chromium, nickel, cobalt, zinc, silver, cadmium, antimony, total mercury, thallium, tin, copper, total arsenic, molybdenum, and lead, were lower than the quantitative limit. The element with the highest migration volume teapot was aluminum, magnesium, and barium; The kettle was aluminum and magnesium; Casserole was aluminum, magnesium, and lithium; The electric stew pot was aluminum. CONCLUSION: This method is easy to operate and has high accuracy, providing an effective and feasible detection method for the determination and evaluation of element migration in Yixing clay pottery.

Modification of bentonite with black cotton soil and carboxyl methyl cellulose for the enhancement of hydraulic performance of geosynthetic clay liners.

Geosynthetic clay liners (GCLs) are mostly used as flow barriers in landfills and waste containments due to their low hydraulic conductivity to prevent the leachate from reaching the environment. The self-healing and swell-shrink properties of soft clays (expansive soils) such as bentonite enable them as promising materials for the GCL core layers. However, it is important to modify their physico-chemical properties in order to overcome the functional limitations of GCL under different hydraulic conditions. In the present study, locally available black cotton soil (BCS) is introduced in the presence of an anionic polymer named carboxymethyl cellulose (CMC) as an alternative to bentonite to enhance the hydraulic properties of GCL under different compositions. The modified GCL is prepared by stitching the liner with an optimum percentage of CMC along with various percentages of BCS mixed with bentonite. Hydraulic conductivity tests were performed on the modified GCL using the flexi-wall permeameter. The results suggest that the lowest hydraulic conductivity of 4.58 × 10-10 m/s is obtained when 25% of BCS is blended with bentonite and an optimum 8% CMC and further addition of BCS results in the reduction of the hydraulic conductivity.

Rare Earth Element Adsorption to Clay Minerals: Mechanistic Insights and Implications for Recovery from Secondary Sources.

The energy transition will have significant mineral demands and there is growing interest in recovering critical metals, including rare earth elements (REE), from secondary sources in aqueous and sedimentary environments. However, the role of clays in REE transport and deposition in these settings remains understudied. This work investigated REE adsorption to the clay minerals illite and kaolinite through pH adsorption experiments and extended X-ray absorption fine structure (EXAFS). Clay type, pH, and ionic strength (IS) affected adsorption, with decreased adsorption under acidic pH and elevated IS. Illite had a higher adsorption capacity than kaolinite; however, >95% adsorption was achieved at pH ∼7.5 regardless of IS or clay. These results were used to develop a surface complexation model with the derived binding constants used to predict REE speciation in the presence of competing sorbents. This demonstrated that clays become increasingly important as pH increases, and EXAFS modeling showed that REE can exist as both inner- and outer-sphere complexes. Together, this indicated that clays can be an important control on the transport and enrichment of REE in sedimentary systems. These findings can be applied to identify settings to target for resource extraction or to predict REE transport and fate as a contaminant.

Electroosmotic flow in soft clay and measures to promote movement under direct current electric field.

Electroosmosis has been proposed as a technique to reduce moisture and thus increase the stability of soft clay. However, its high energy consumption and uneven reinforcement effect has limited its popularization and application in practical engineering. This paper presents the results of some electrokinetic tests performed on clayey specimens with different electrification time and anode boundary conditions. The results indicate that the timing of the formation of electroosmotic flow (EF) by the water originally contained in different soil cross sections, from the anode to the cathode, varies. The measuring soil cross section nearest the anode first reached the limiting water content of 22%±3% and electroosmosis had to be stopped. Water injection into the anode during electroosmosis enhanced further drainage of other four measuring soil cross sections until the second soil cross section from the anode reached the limiting water content of 30%±2%. Electroosmosis with water injection into the anode technique provides more uniform reinforcement, increasing EF, and environmental protection. The experimental results highlighted the relevant and expected contribution of water injection into the anode on the effectiveness of the electroosmotic treatment as a soft clay improvement technique.

Investigating the impact of soil properties, application rates and environmental conditions on pyroxasulfone dissipation and its ecotoxicological effects on soil health in aridisols of Punjab.

This study is to understand the fate and ecological consequences of pyroxasulfone in aridisols of Punjab, a detailed dissipation study in soil, its influence on soil enzymes, microbial count and succeeding crops was evaluated. Half-lives (DT50) increased with an increase in the application rate of pyroxasulfone. Dissipation of pyroxasulfone decreased with increase in organic matter content of soil and was slower in clay loam soil (DT50 12.50 to 24.89) followed by sandy loam (DT50 8.91 to 17.78) and loamy sand soil (DT50 6.45 to 14.89). Faster dissipation was observed under submerged conditions (DT50 2.9 to 20.99 days) than under field capacity conditions (DT50 6.45 to 24.89 days). Dissipation increased with increase in temperature with DT50 varying from 6.46 to 24.88, 4.87 to 22.89 and 2.97 to 20.99 days at 25 ± 2, 35 ± 2 and 45 ± 2 °C, respectively. Dissipation was slower under sterile conditions and about 23.87- to 33.74-fold increase in DT50 was observed under sterile conditions as compared to non-sterile conditions. The application of pyroxasulfone showed short-lived transitory effect on dehydrogenase, alkaline phosphatase and soil microbial activity while herbicide has non-significant effect on soil urease activity. PCA suggested that dehydrogenase and bacteria were most sensitive among enzymatic and microbial activities. In efficacy study, pyroxasulfone effectively controlled Phalaris minor germination, with higher efficacy in loamy sand soil (GR50 2.46 µg mL-1) as compared to clay loam soil (GR50 5.19 µg mL-1).

Evaluating the agronomic efficiency of alternative phosphorus sources applied in Brazilian tropical soils.

Understanding the efficacy of alternative phosphorus (P) sources in tropical soils is crucial for sustainable farming, addressing resource constraints, mitigating environmental impact, improving crop productivity, and optimizing soil-specific solutions. While the topic holds great importance, current literature falls short in providing thorough, region-specific studies on the effectiveness of alternative P sources in Brazilian tropical soils for maize cultivation. Our aim was to assess the agronomic efficiency of alternative P sources concerning maize crop (Zea mays L.) attributes, including height, shoot dry weight, stem diameter, and nutrient accumulation, across five Brazilian tropical soils. In greenhouse conditions, we carried out a randomized complete block design, investigating two factors (soil type and P sources), evaluating five tropical soils with varying clay contents and three alternative sources of P, as well as a commercial source and a control group. We evaluated maize crop attributes such as height, dry weight biomass, and nutrient accumulation, P availability and agronomic efficiency. Our results showed that, although triple superphosphate (TSP) exhibited greater values than alternative P sources (precipitated phosphorus 1, precipitated phosphorus 2 and reactive phosphate) for maize crop attributes (e.g., height, stem diameter, shoot dry weight and phosphorus, nitrogen, sulfur, calcium and magnesium accumulation). For instance, PP1 source increased nutrient accumulation for phosphorus (P), nitrogen (N), and sulfur (S) by 37.05% and 75.98% (P), 34.39% and 72.07% (N), and 41.94% and 72.69% (S) in comparison to PP2 and RP, respectively. Additionally, PP1 substantially increased P availability in soils with high clay contents 15 days after planting (DAP), showing increases of 61.90%, 99.04%, and 38.09% greater than PP2, RP, and TSP. For Ca and Mg accumulation, the highest values were found in the COxisol2 soil when PP2 was applied, Ca = 44.31% and 69.48%; and Mg = 46.23 and 75.79%, greater than PP1 and RP, respectively. Finally, the highest values for relative agronomic efficiency were observed in COxisol2 when PP1 was applied. The precipitated phosphate sources (PP1 and PP2) exhibited a similar behavior to that of the commercial source (TSP), suggesting their potential use to reduce reliance on TSP fertilization, especially in soils with low clay contents. This study emphasized strategies for soil P management, aimed at assisting farmers in enhancing maize crop productivity while simultaneously addressing the effectiveness of alternative P sources of reduced costs.

Evidence for immune activation in pathogenesis of the HLA class II associated disease, podoconiosis.

Available evidences suggest that podoconiosis is triggered by long term exposure of bare feet to volcanic red clay soil particles. Previous genome-wide studies in Ethiopia showed association between the HLA class II region and disease susceptibility. However, functional relationships between the soil trigger, immunogenetic risk factors and the immunological basis of the disease are uncharted. Therefore, we aimed to characterise the immune profile and gene expression of podoconiosis patients relative to endemic healthy controls. Peripheral blood immunophenotyping of T cells indicated podoconiosis patients had significantly higher CD4 and CD8 T cell surface HLA-DR expression compared to healthy controls while CD62L expression was significantly lower. The levels of the activation markers CD40 and CD86 were significantly higher on monocytes and dendritic cell subsets in patients compared to the controls. RNA sequencing gene expression data indicated higher transcript levels for activation, scavenger receptors, and apoptosis markers while levels were lower for histones, T cell receptors, variable, and constant immunoglobulin chain in podoconiosis patients compared to healthy controls. Our finding provides evidence that podoconiosis is associated with high levels of immune activation and inflammation with over-expression of genes within the pro-inflammatory axis. This offers further support to a working hypothesis of podoconiosis as soil particle-driven, HLA-associated disease of immunopathogenic aetiology.

Shear strength and particle breakage of construction and demolition waste as a function of moisture state and compaction level: Insights for sustainable highway engineering.

In this study, the variation of shear strength behavior and particle breakage (after shearing), as a function of moisture state and compaction level, is investigated for recycled concrete aggregate blended with recycled clay masonry. Recycled masonry was blended with concrete aggregate in percentages ranging from 0% to 30% by total weight. Tests include; basic engineering characteristics (particle size, modified compaction, hydraulic conductivity, and California Bearing Ratio, CBR) as well as unconsolidated undrained static triaxial testing. In triaxial tests, moisture levels ranged from 60% to 100% of optimum moisture content, but compaction levels ranged from 90% to 98% of maximum dry density. The hydraulic conductivity for blends is approximately 2x10-6 cm/s, which indicates a relatively low hydraulic conductivity. Results show a proportional linear relationship between the shear strength of blends and the level of compaction. Despite this, both apparent cohesion and shear strength exhibited reverse linear trends. As expected, more compaction effort resulted in more particle breakage. Strict control should be performed over the compaction process to achieve the required compaction level which resulting in pavement materials being stiffer.

Deciphering silver nanoparticles perturbation effects and risks for soil enzymes worldwide: Insights from machine learning and soil property integration.

Globally extensive research into how silver nanoparticles (AgNPs) affect enzyme activity in soils with differing properties has been limited by cost-prohibitive sampling. In this study, customized machine learning (ML) was used to extract data patterns from complex research, with a hit rate of Random Forest > Multiple Imputation by Chained Equations > Decision Tree > K-Nearest Neighbors. Results showed that soil properties played a pivotal role in determining AgNPs' effect on soil enzymes, with the order being pH > organic matter (OM) > soil texture ≈ cation exchange capacity (CEC). Notably, soil enzyme activity was more sensitive to AgNPs in acidic soil (pH < 5.5), while elevated OM content (>1.9 %) attenuated AgNPs toxicity. Compared to soil acidification, reducing soil OM content is more detrimental in exacerbating AgNPs' toxicity and it emerged that clay particles were deemed effective in curbing their toxicity. Meanwhile sand particles played a very different role, and a sandy soil sample at > 40 % of the water holding capacity (WHC), amplified the toxicity of AgNPs. Perturbation mapping of how soil texture alters enzyme activity under AgNPs exposure was generated, where soils with sand (45-65 %), silt (< 22 %), and clay (35-55 %) exhibited even higher probability of positive effects of AgNPs. The average calculation results indicate the sandy clay loam (75.6 %), clay (74.8 %), silt clay (65.8 %), and sandy clay (55.9 %) texture soil demonstrate less AgNPs inhibition effect. The results herein advance the prediction of the effect of AgNPs on soil enzymes globally and determine the soil types that are more sensitive to AgNPs worldwide.

Pre-emergence herbicides widely used in urban and farmland soils: fate, and potential human and environmental health risks.

We determined the distribution, fate, and health hazards of dimethenamid-P, metazachlor, and pyroxasulfone, the effective pre-emergence herbicides widely used both in urban and agricultural settings globally. The rate-determining phase of sorption kinetics of these herbicides in five soils followed a pseudo-second-order model. Freundlich isotherm model indicated that the herbicides primarily partition into heterogeneous surface sites on clay minerals and organic matter (OM) and diffuse into soil micropores. Principal component analysis revealed that soil OM (R2, 0.47), sand (R2, 0.56), and Al oxides (R2, 0.33) positively correlated with the herbicide distribution coefficient (Kd), whereas clay (R2, ‒ 0.43), silt (R2, ‒ 0.51), Fe oxides (R2, ‒ 0.02), alkaline pH (R2, ‒ 0.57), and EC (R2, ‒ 0.03) showed a negative correlation with the Kd values. Decomposed OM rich in C=O and C-H functional groups enhanced herbicide sorption, while undecomposed/partially-decomposed OM facilitated desorption process. Also, the absence of hysteresis (H, 0.27‒0.88) indicated the enhanced propensity of herbicide desorption in soils. Leachability index (LIX, < 0.02-0.64) and groundwater ubiquity score (GUS, 0.02‒3.59) for the soils suggested low to moderate leaching potential of the herbicides to waterbodies, indicating their impact on water quality, nontarget organisms, and food safety. Hazard quotient and hazard index data for human adults and adolescents suggested that exposure to soils contaminated with herbicides via dermal contact, ingestion, and inhalation poses minimal to no non-carcinogenic risks. These insights can assist farmers in judicious use of herbicides and help the concerned regulatory authorities in monitoring the safety of human and environmental health.

Gypsum amendment of agricultural fields to decrease phosphorus losses - Evidence on a catchment scale.

Amending agricultural fields with gypsum has been proposed as a cost-effective measure to reduce P load on coastal waters. We treated 1490 ha of clayey fields with phosphogypsum (4 t ha-1) in Southwest Finland and monitored the recipient river with online sensors and water sampling for the preceding spring and 5 years after the amendment. Gypsum immediately decreased the riverine fluxes, the effect lasting at least 5 years for particulate P (PP), total suspended solids (TSS), and dissolved organic C (DOC) and 1-2 years for dissolved reactive P (DRP). Compared with an upstream control area, the fluxes of PP, TSS, and DOC decreased by 15%, 25%, and 8.9%, respectively, as a 5-year average. Assuming the change in the fluxes occurred only due to gypsum, the amended fields showed 35%, 59%, and 64% lower losses for PP, TSS, and DOC than the unamended ones. More than half of the gypsum remained in the soil even after 5 years; thus, although the efficiency of gypsum lessened over time, its residual effect may be present. However, the difference in the erodibility between the control and treatment areas impacted the validity of the results, especially as the pre-gypsum period was short. In addition, the performance of gypsum showed spatial variation.

Investigation of the interface of fungal mycelium composite building materials by means of low-vacuum scanning electron microscopy.

Low-vacuum scanning electron microscopy (low-vacuum SEM) is widely used for different applications, such as the investigation of noncoated specimen or the observation of biological materials, which are not stable to high vacuum. In this study, the combination of mineral building materials (concrete or clay plaster) with a biological composite (fungal mycelium composite) by using low-vacuum SEM was investigated. Fungal biotechnology is increasingly gaining prominence in addressing the challenges of sustainability transformation. The construction industry is one of the biggest contributors to the climate crises and, therefore, can highly profit from applications based on regenerative fungal materials. In this work, a fungal mycelium composite is used as alternative to conventional insulating materials like Styrofoam. However, to adapt bio-based products to the construction industry, investigations, optimisations and adaptations to existing solutions are needed. This paper examines the compatibility between fungal mycelium materials with mineral-based materials to demonstrate basic feasibility. For this purpose, fresh and hardened concrete specimens as well as clay plaster samples are combined with growing mycelium from the tinder fungus Fomes fomentarius. The contact zone between the mycelium composite and the mineral building materials is examined by scanning electron microscopy (SEM). The combination of these materials proves to be feasible in general. The use of hardened concrete or clay with living mycelium composite appears to be the favoured variant, as the hyphae can grow into the surface of the building material and thus a layered structure with a stable connection is formed. In order to work with the combination of low-density organic materials and higher-density inorganic materials simultaneously, low-vacuum SEM offers a suitable method to deliver results with reduced effort in preparation while maintaining high capture and magnification quality. Not only are image recordings possible with SE and BSE, but EDX measurements can also be carried out quickly without the influence of a coating. Depending on the signal used, as well as the magnification, image-recording strategies must be adapted. Especially when using SE, an image-integration method was used to reduce the build-up of point charges from the electron beam, which damages the mycelial hyphae. Additionally using different signals during image capture is recommended to confirm acquired information, avoiding misinterpretations.

Microbial mechanisms in nitrogen fertilization: Modulating the re-mobilization of clay mineral-bound cadmium in agricultural soils.

Soil cadmium (Cd) can affect crop growth and food safety, and through the enrichment in the food chain, it ultimately poses a risk to human health. Reducing the re-mobilization of Cd caused by the release of protons and acids by crops and microorganisms after stabilization is one of the significant technical challenges in agricultural activities. This study aimed to investigate the re-mobilization of stabilized Cd within the clay mineral-bound fraction of soil and its subsequent accumulation in crops utilizing nitrogen ammonium nitrogen (NH4+-N) and nitrate nitrogen (NO3--N), at 60 and 120 mg kg-1. Furthermore, the study harvested root exudates at various growth stages to assess their direct influence on the re-mobilization of stabilized Cd and to evaluate the indirect effects mediated by soil microorganisms. The results revealed that, in contrast to the NO3--N treatment, the NH4+-N treatment significantly enhanced the conversion of clay mineral-bound Cd in the soil to NH4NO3-extractable Cd. It also amplified the accumulation of Cd in edible amaranth, with concentrations in roots and shoots rising from 1.7-6.0 mg kg-1 to 4.3-9.8 mg kg-1. The introduction of NH4+-N caused a decrease in the pH value of the rhizosphere soil and stimulated the production and secretion organic and amino acids, such as oxalic acid, lactic acid, stearic acid, succinic acid, and l-serine, from the crop roots. Furthermore, compared to NO3--N, the combined interaction of root exudates with NH4+-N has a more pronounced impact on the abundance of microbial genes associated with glycolysis pathway and tricarboxylic acid cycle, such as pkfA, pfkB, sucB, sucC, and sucD. The effects of NH4+-N on crops and microorganisms ultimately result in a significant increase in the re-mobilization of stabilized Cd. However, the simulated experiments showed that microorganisms only contribute to 3.8-6.6 % of the re-mobilization of clay mineral-bound Cd in soil. Therefore, the fundamental strategy to inhibit the re-mobilization of stabilized Cd in vegetable cultivation involves the regulation of proton and organic acid secretion by crops.

A comparative study on the Cs adsorption/desorption and structural changes in different clay minerals.

We investigated the structural changes in clay minerals after Cs adsorption and understood their low desorption efficiency using an ion-exchanger. We focused on the role of interlayers in Cs adsorption and desorption in 2:1 clay minerals, namely illite, hydrobiotite, and montmorillonite, using batch experiments and XRD and EXAFS analyses. The adsorption characteristics of the clay minerals were analyzed using cation exchange capacity (CEC), maximum adsorption isotherms (Qmax), and radiocesium interception potential (RIP) experiments. Although illite showed a low CEC value, it exhibited high selectivity for Cs with a relatively high RIP/CEC ratio. The Cs desorption efficiency after treatment with a NaCl ion exchanger was the highest for illite (74.3%), followed by hydrobiotite (45.5%) and montmorillonite (30.3%); thus, Cs adsorbed onto planar sites, rather than on interlayers or frayed edge sites (FESs), is easily desorbed. After NaCl treatment, XRD analysis showed that the low desorption efficiency was due to the collapse of the interlayer-fixed Cs, which tightly narrowed the interlayers' hydrobiotite due to the ion exchange of divalent cations (Mg2+ or Ca2+) into the monovalent cation (Na+). Moreover, EXAFS analysis showed that hydrobiotite formed inner-sphere structures after NaCl desorption, indicating that it was difficult to remove Cs from NaCl desorption due to the collapsed hydrobiotite and montmorillonite interlayers as well as the strong bonding in FESs of illite. In contrast, chelation desorption using oxalic acid effectively dissolved the narrowed interlayers of hydrobiotite (98%) and montmorillonite (85.26%), enhancing the desorption efficiency. Therefore, low desorption efficiency for Cs clays using an ion exchanger was caused by the collapsed interlayer due to the exchange between monovalent cation and divalent cation.

Using L. minor and C. elegans to assess the ecotoxicity of real-life contaminated soil samples and their remediation by clay- and carbon-based sorbents.

Toxic substances, such as polycyclic aromatic hydrocarbons (PAHs) and heavy metals, can accumulate in soil, posing a risk to human health and the environment. To reduce the risk of exposure, rapid identification and remediation of potentially hazardous soils is necessary. Adsorption of contaminants by activated carbons and clay materials is commonly utilized to decrease the bioavailability of chemicals in soil and environmental toxicity in vitro, and this study aims to determine their efficacy in real-life soil samples. Two ecotoxicological models (Lemna minor and Caenorhabditis elegans) were used to test residential soil samples, known to contain an average of 5.3, 262, and 9.6 ppm of PAHs, lead, and mercury, for potential toxicity. Toxicity testing of these soils indicated that 86% and 58% of soils caused ≤50% inhibition of growth and survival of L. minor and C. elegans, respectively. Importantly, 3 soil samples caused ≥90% inhibition of growth in both models, and the toxicity was positively correlated with levels of heavy metals. These toxic soil samples were prioritized for remediation using activated carbon and SM-Tyrosine sorbents, which have been shown to immobilize PAHs and heavy metals, respectively. The inclusion of low levels of SM-Tyrosine protected the growth and survival of L. minor and C. elegans by 83% and 78%, respectively from the polluted soil samples while activated carbon offered no significant protection. These results also indicated that heavy metals were the driver of toxicity in the samples. Results from this study demonstrate that adsorption technologies are effective strategies for remediating complex, real-life soil samples contaminated with hazardous pollutants and protecting natural soil and groundwater resources and habitats. The results highlight the applicability of these ecotoxicological models as rapid screening tools for monitoring soil quality and verifying the efficacy of remediation practices.

Simultaneously decreasing arsenic and cadmium in rice by soil sulfate and limestone amendment under intermittent flooding.

Water management in paddy soils can effectively reduce the soil-to-rice grain transfer of either As or Cd, but not of both elements simultaneously due to the higher mobility of As under reducing and Cd under oxidizing soil conditions. Limestone amendment, the common form of liming, is well known for decreasing Cd accumulation in rice grown on acidic soils. Sulfate amendment was suggested to effectively decrease As accumulation in rice, especially under intermittent soil flooding. To study the unknown effects of combined sulfate and limestone amendment under intermittent flooding for simultaneously decreasing As and Cd in rice, we performed a pot experiment using an acidic sandy loam paddy soil. We also included a clay loam paddy soil to study the role of soil texture in low-As rice production under intermittent flooding. We found that liming not only decreased rice Cd concentrations but also greatly decreased dimethylarsenate (DMA) accumulation in rice. We hypothesize that this is due to suppressed sulfate reduction, As methylation, and As thiolation by liming in the sulfate-amended soil and a higher share of deprotonated DMA at higher pH which is taken up less readily than protonated DMA. Decreased gene abundance of potential soil sulfate-reducers by liming further supported our hypothesis. Combined sulfate and limestone amendment to the acidic sandy loam soil produced rice with 43% lower inorganic As, 72% lower DMA, and 68% lower Cd compared to the control soil without amendment. A tradeoff between soil aeration and water availability was observed for the clay loam soil, suggesting difficulties to decrease As in rice while avoiding plant water stress under intermittent flooding in fine-textured soils. Our results suggest that combining sulfate amendment, liming, and intermittent flooding can help to secure rice safety when the presence of both As and Cd in coarse-textured soils is of concern.

Clean and accurate soil quality monitoring in mining areas under environmental rehabilitation in the Eastern Brazilian Amazon.

Soil quality monitoring in mining rehabilitation areas is a crucial step to validate the effectiveness of the adopted recovery strategy, especially in critical areas for environmental conservation, such as the Brazilian Amazon. The use of portable X-ray fluorescence (pXRF) spectrometry allows a rapid quantification of several soil chemical elements, with low cost and without residue generation, being an alternative for clean and accurate environmental monitoring. Thus, this work aimed to assess soil quality in mining areas with different stages of environmental rehabilitation based on predictions of soil fertility properties through pXRF along with four machine learning algorithms (projection pursuit regression, PPR; support vector machine, SVM; cubist regression, CR; and random forest, RF) in the Eastern Brazilian Amazon. Sandstone and iron mines in different chronological stages of rehabilitation (initial, intermediate, and advanced) were evaluated, in addition to non-rehabilitated and native forest areas. A total of 81 soil samples (26 from sandstone mine and 55 from iron mine) were analyzed by both traditional wet-chemistry methods and pXRF. The available/exchangeable contents of K, Ca, B, Fe, and Al, in addition to H+Al, cation exchange capacity at pH = 7, Al saturation, soil organic matter, pH, sum of bases, base saturation, clay, and sand were accurately predicted (R2 > 0.70) using pXRF data, with emphasis on the prediction of Fe (R2 = 0.93), clay content (R2 = 0.81), H+Al (R2 = 0.81), and K+ (R2 = 0.85). The best predictive models were developed by RF and CR (86%) and when considering pXRF data + mining area + stage of rehabilitation (73%). The results highlight the potential of pXRF to accurately assess soil properties in environmental rehabilitation areas in the Amazon region (yet scarcely evaluated under this approach), promoting a more agile and cheaper preliminary diagnosis compared to traditional methods.

Mitigating phthalate toxicity: The protective role of humic acid and clay in zebrafish larvae.

This research study aimed to explore the mitigating effects of humic acid and clay on the toxicity induced by three different phthalates (DBP, DEP, DEHP) on zebrafish larvae growth. Prolonged exposure to DBP resulted in a concerning 87.33% mortality rate, significantly reduced to 7.3% when co-administered with humic acid. A similar reduction in mortality was observed for the other two phthalates (DEP and DEHP). Additionally, the introduction of phthalates with humic acid, clay, or their combination led to a significant decrease in the malformation rate in larvae. High-Performance Liquid Chromatography (HPLC) analysis of phthalates in treatments revealed a noteworthy decline in their concentration when combined with humic acid and clay. This suggests a reduced bioavailability of phthalates to larvae, aligning with diminished toxicity, lower mortality, fewer malformations, and improved organ development, as well as less oxidative stress. Furthermore, measurements of larval length and morphological scoring affirmed the protective role of humic acid and clay in promoting the normal growth of zebrafish. This study underscores the potential of environment modulators, such as humic acid and clay, as effective bioremediation agents against phthalate toxicity. The generation of reactive oxygen species (ROS), indicative of oxidative stress, was markedly higher in larvae treated solely with phthalates compared to the control. Conversely, larvae treated with a combination of phthalates and humic acid or clay exhibited a significant decrease in ROS generation, signaling a decline in oxidative stress. Histopathological analysis of adult fish subjected to various treatments revealed significant damage to vital organs like the liver and intestine when treated with phthalates alone. However, when phthalates were introduced with humic acid, clay, or both, the morphology closely resembled that of the control, reinforcing the protective role of humic acid and clay in zebrafish development against administered phthalates.

Systematic review of alternative materials that improve retention of potentially toxic metals in soil/clay liners in waste disposal areas.

When soils available for the construction of liners do not display the characteristics necessary for a good performance, mixtures with other materials can be employed for achieving the desired quality. Several researchers have addressed those mixtures from either a geotechnical or a gas diffusion perspective, emphasizing low hydraulic conductivity. However, in recent years, growing attention has been drawn to the ability of liners to mitigate contamination. The literature lacks studies on the use of amendments for soil liners or cover systems to retain potentially toxic metals, which are important inorganic contaminants. This paper provides a systematic review of the literature considering publications available on Web of Science and SpringerLink databases between January 1st, 2012, and December 5th, 2022. The aim of the review was to identify the types of soils and amendments studied as liners or cover systems for such retention of potentially toxic metals, the methodologies of application of the alternative materials in the soils, and the research gaps and perspectives in the field. Seventeen papers that addressed 31 materials as amendments were retrieved. The most studied amendment was coal fly ash, and 17 amendments were residues or by-products, which indicates concerns over waste destination and sustainability. Among the potentially toxic metals analyzed are Pb, Cu, and Cd. Gaps such as lack of pilot, field-scale, and long-term studies, as well as perspectives for future research (e.g., different liner configurations, concomitant mixtures of two or more materials in the soil, and focus on the sustainability of amendments), were identified.